Abstract:

The influence of different lysine concentration on the cationic amino acid transporter mRNA expression porcineIntestinal epithelial cells (IEC) in vitro was studied by the primary culture of porcine IEC. The primary culture of porcine IEC system was established by mechanical and enzymatic (collagenaseⅡ) methods using small intestines removed from colostrum-deprived newborn piglets. Total RNA was isolated from IEC samples after treatment with 0.5 mM, 2mM and 8mM lysine for 96 hours. The cationic amino acid transporters mRNA expression abundances were determined by real-time Q–PCR. The results showed as follows: High concentrations of lysine have significant up-regulation effect on the CAT-1 mRNA expression and there was an obvious dose-dependent effect (P<0.01). Lysine treatment had no distinct effect on the CAT-2 mRNA expression abundance. CAT-2 mRNA expressions of 8mM and 2mM lysine treatment were lower than that of 0.5mM lysine group, but with no significant difference (P>0.05). Lysine treatment had no significant effect on the y+LAT1 mRNA expression abundance (P>0.05). High concentrations of lysine could enhance 4F2hc mRNA expression. 4F2hc mRNA expression abundances of 0.5 mM and 2mM lysine groups were significantly lower than that of 8 mM lysine group (P<0.05). There was no significant difference between 0.5mM and 2mM group (P>0.05).