Abstract:

Detection of ASF infection in CSF epidemiological area of HeNan province; Methods: Suspected- CSF histologic materials of 27 pig farms in HeNan were detected and identified by the commercial antigen-capture ELISA assay and RT-PCR for Detection and Differentiation of Wild-type and Vaccine Viruses of Classical Swine Fever Virus (CSFV) . At the same time, Primers were designed according to the nucleotide sequence of VP73 gene of African swine fever virus(ASFV) and PCR assays were performed to Screen ASF infection; Result: None ASF infection were found in CSF epidemiological area of HeNan province; The detection rate of CSFV by RT- PCR was higher than that by the commercial antigen-capture ELISA assay, the difference was significant. Results showed that none ASF infection was found in HeNan province, though ASFV threat was existed in China as a neighboring country of Russia now.