Abstract:

CORYNE (CRN) plays important roles in stem cell division and differentiation of shoot apical meristem (SAM). In this study, we have investigated the peptides interacting with the intracellular protein kinase domain of CRN by using the 7-mer phage display library. By using CRN as a ligand, phage clones binding to it were screened from the library through 4 rounds of biopanning. After sequencing and measuring binding activity by ELISA, a clone specific binding to the intracellular protein kinase domain of CRN has been selected, and the encoding peptide sequence was TLTELVR, which was considered to contain the key peptide sequence interacting with the intracellular protein kinase domain of CRN.