Abstract:

Calluses and dihaploid plantlets were obtained from unpollinated ovaries of Qingshu168 and Qingshu7 by the in vitro culture. The buds were preprocessed at 4℃ for 24-72 h, and their callus induction rate was remarkably increased, comparing with that of non-pretreatment. The most suitable differentiation medium for Qingshu168 was MS+2-4-D 2.0 mg/L+ZT 0.1 mg/L+Sucrose 30 g/L+agar 7 g/L, and for Qingshu7 was 1/2 MS+GA3 0.5 mg/L+2-4-D 0.5 mg/L+KT 2.0 mg/L+BAP 2.0 mg/L+ ZT 0.2 mg/L+sucrose 20 g/L+agar 7 g/L. It appeared that the plantlet differentiation mainly depended on cultivars.