Abstract:

Arabidopsis thaliana leaves were labeled by fluorescent probe Fluo-3/AM under low temperature at 4 ℃ to measure the fluorescent intensity of intracellular Ca2+ which was pretreated with heparin on jasmonic acid (JA)-induced. The results showed that the fluorescent intensity of [Ca2+]cyt was reduced after pretreated with different concentration of heparin, and then treated with 100 μmol/L JA, the fluorescent intensity of [Ca2+]cyt was close to the fluorescent intensity which was not pretreated with heparin. The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca2+ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.