Abstract:

Abstract: Among the 7 tested fungal elicitors, a fungal elicitor from Rhizopus oryzae extract could achieve highest total camptothecin production with suspension cultures of Camptotheca acuminata Decaisne when the elicitor at 30 mg/L of final concentration was supplemented on the 18th day of growth. Among the 4 tested anti-browning agents, when phytic acid at 2 g/L of final concentration was supplemented with suspension cultures on the 15th day of growth, the highest dry cell weight and total camptothecin production were achieved. When 2 g/L phytic acid was supplemented on the 15th day and 30 mg/L Rhizopus oryzae extract as the fungal elicitor was supplemented afterward on the 18th day into C. acuminata cell suspension cultures, maximum cell dry weight was achieved, reaching 26.9 g/L on day 24, which was 1.2-folds of the untreated cultures (its highest production was achieved on the 20th day). Also, maximum total camptothecin production of double treated cultures was achieved, reaching 34.2 mg/L on day 26, which was 9.0-folds of the untreated cultures (its highest production was achieved on the 20th day).