Abstract:

Sorghum traditional tissue culture technology was still used in inducing somaclonal variations, which meant the somaclonal variations occurred naturally and the frequency of variations was not very high, thus restricting the application effectiveness. This study aimed to enhance frequency of variations through the artificial chemical induction of somaclonal variations. The test materials used in the study were R-line San Chi San and Jin liang no 5. The chemical mutagen was Diethyl sulphate. Generally, the chemical mutagen was poisonous to organisms and therefore it was important to establish safty treatment procedures without pollution. Through years of research and exploration, safety and pollution-free Procedure for treating callus with toxic chemicals was established. The results showed that 0.5%-1% concentrations of DES was suitable to treat sorghum calli.