Abstract:

For optimizing SRAP-PCR reaction system of Miscanthus sinensis, the single factor experiment included three factors, such as Taq DNA polymerse, dNTPs and Mg2+, were optimized by using the total genomic DNA in this research. In conclusion the reaction system was obtained as below：1 μL 10xTaq Buffer, 0.5 mmol/L dNTPs,0.6 U Taq DNA polymerase, 2 mmol/L MgCl2, 20 ng DNA，0.8 μmol／L primer and with total 10 μL reaction solution．This result provided a useful technique for the genetic variation research and molecular marker-assisted breeding of M. sinensis.