Abstract:

In order to make DHV antigens,the experiment utilize IFA and CFA. The BALB/c mice were immunized with DHV for three times . The spleen’s B lymphocyte of the mice should be fused with SP2/0 plasmacytoma cells until the immunopotency was up to 1:10000.The masccline hybridoma cells were screened by indirect enzyme-link immunosorbent assay. Hybridomas whose supernatants show stronger positive were subcloned by limited dilution methods for 3~4 times, until the positive rate come up to 100%.It was named 2E3. The immunopotency of ascites and supernatant was 10×25,100×26..And the subtype of 2E3 was IgGl.