Abstract:

WRKY protein play an important role both in biotic and abiotic stress, also in growth and development of plant. In order to study the function of WRKY in tomato, the primers were designed according to the cloned WRKY transcription factors. The 608 bp fragment was obtained from tomato with RT-PCR method. Sequential analysis indicated that this sequence contained WRKYGQK conservative domain, and the similarity with Capsicum annuum WRKY-c and Nicotiana tabacum NtWRKY-7 were 79% and 74%, respectively. The results showed that WRKY gene sequence in tomato was cloned successfully.