Abstract:

Abstract: 【OBJECTIVE】To standardize the method to induce and cultivate swine blood-derived dendritic cells( DC) in vitro, which could provide a technology platform for the study of related-viral diseases’ mechanism and making the measures of their prevention and treatment. 【METHODS】Porcine peripheral blood mononuclear cells (PBMCs) were separated. The adherent cells of PBMCs on the flask were induced by GM-CSF and IL-4 and their shapes were observed by the optical microscope at different times. After 7 day, these induced cells were collected and their form and surface markers CD1a&SWC3a were identified by light microscope, flow cytometry and laser confocal microscope. The MHC-II and CD80/86 molecules on the surface of these cells were identified by bicolor flow cytometry. 【RESULTS】The results showed that the induced cells were dendritic, double-positive rates of CD1a/SWC3a and MHC-II and CD80/86 were 90.1% and 98.3% respectively. Colony-like cells were observed and the surface marker CD1a/SWC3a were double-positive under laser confocal microscope.【CONCLUSION】The swine blood-derived DCs were induced successfully according to the analysis of phenotype and morphology. The standard procedure of the induction is acquired, which has laid a foundation for the research of immuno-suppression mechanism of porcine viral diseases that the target cells are DCs.