Abstract:

In order to save the application amount of pure pollen and reduce seed production costs, the effects of artificial pollination using anther medium pollen on the fruit setting percentage and seed quality of MS Yunyan 85, MS Yunyan 87 and MSK326 were investigated. Pure pollen from Yunyan 85, Yunyan 87 and K326 diluted with its anther powder at the proportion of 2:1, 1:1, 1:2 and 1:3(pollen:anther powder v/v) respectively, using pure pollen as control. The results showed that the effects of artificial pollination of anther medium pollen (2:1, pollen:anther powder v/v) was better than other treatments for MS Yunyan 85, MS Yunyan 87 and MSK326, fruit setting percentage, seed number per capsule, 1000-seed weight, germination energy, germination percentage, germination index, average germination time and yield per plant were no significant difference compared to the control, the application amount of pure pollen was decreased by 33.33%. The results indicated that the suitable proportion of anther medium pollen could be used in tobacco seed multiplication, could save the application amount of pure pollen and reduce seed production costs.