Abstract:

Abstract: 【OBJECTIVE】In order to clarify the mechanisms of melatonin affected goat reproduction traits, and compared the different expressed amount of melatonin receptors (MTR1A) in ovary tissues between Jining Grey Goat with high superfoetation and Yimeng Black Goat with single birth.【METHOD】Real-time fluorescent quantitate differential display PCR technique were used to detected the expressed amount of melatonin receptors (MTR1A) in ovary tissure of Jining Gray Goat and Yimeng Black Goat in oestrus stage, while GAPDH internal standards were used as control. 【RESULTS】The results showed that MTR1A mRNA were expressed in ovary tissue of both goats, and the expressed amount of MTR1A mRNA was lower at Jining Gray Goat ovary than that of it at Yimeng Black Goat ovarys. CONCLUSION】The results suggest that MTR1A mRNA are expressed in both goat ovary obviously, the differential expression of MTR1A may affect the development, maturity and ovulating of goat ovary. This provided the molecular genetic data to clarity the mechanism of MTR1A action at goat reproduction.