Abstract: CSFV gene vaccine was constructed by inserting full-length cDNA of CSFV Shimen strain E2 gene into an eukaryotic expression vector pcDNA4.0. The recombinant plasmid was transfected into eukaryotic cells 293T by calcium phosphate transfection method and transient expressive E2 envelope protein was analyzed by FACS. BALB/c mice were intramuscular injection with the recombinant plasmid. Anti-CSFV E2 antibody was screened by FACS and ELISA. The results showed that CSFV E2 protein was expressed transciently in 293T cells. Specific anti-E2 antibody could be detected in DNA immune mouse serum by FACS and ELISA..