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Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (3): 116-123.

Special Issue: 生物技术 油料作物 棉花

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Effects of the Transferred Resist-diseases Genes(Chi+Glu) to the Biological Characteristics of Cotton

  

  • Received:2010-07-13 Revised:2010-08-12 Online:2011-02-05 Published:2011-02-05

Abstract:

These researches were studied in order to establish the environmental safety assessment techniques of resistant transgenic cotton in China. Test material was the cotton which was transferred the chitinase and glucanase bivalent disease-resistance gene (Chi+Glu) to, the contrast materials were receptor China Cotton Research Instituteand 24(CCRI 24) and Ganmian 11. Observed and compared the reproductive traits, yield traits, biological characteristics of above-mentioned materials. The results showed that growth periods of disease-resistance cotton were extended 11 days and 3 days respectively, compared with CCRI 24 and Ganmian 11.By least significant difference LSD test, the double disease-resistant gene (Chi+Glu) made the height of cotton grow slowly, the influence of that was significant in the seeding stage and notable in the bud stage, but the growth was faster in boll stage and the effect was significant. The bell, flower and bud growth retardation, the first fruit branch forward, and these influence were significant. While some effects were very significantly ,included that the height of the first fruiting branch reduced, ruit branch pitch widening, seed cotton and lint yield raised, the germination rate increased (upward 13%).The influence to the following traits were well, including boll weight, seed cotton weight, lint weight, Seed index, lint index, the number of sterile seeds and lint percentage. Boll shell weight, the weight of sterile seeds were worse. Some indexes of cotton fibers were also effected notablely, for example, the upper half of cotton fibers was shorter, uniformity index and micronaire were increased, elongatio and breaking strength were decreased, spinning consistency index was lower. The test conclusion showed that the effects of the Chi and Glu gene to the above biological characteristics of cotton were significant and very significant, particularly on the impact of yield traits were better significantly (seed cotton yield increased 1491.9 kg/hm2).