Abstract:

Abstract: In this paper，the screening and purification technology of astaxanthin was studied, which would be the important basis for its character research and value in use. After extracting and concentrating，astaxanthin crude extract was saponified by NaOH-Ethanol. Then the astaxanthin was purified by polyamide column chromatography, and its purification efficiency tested by TLC and HPLC. The results showed that the optimum saponification conditions were: Saponification liquid 20 g/L NaOH-Ethanol，temperature 15℃，Saponification time 10 min. The astaxanthin sample was puried by polyamide colum chromatography under N-Hexane, N-Hexane:Dichloromethane=4:1, N-Hexane:Dichloromethane=2:1, dichloromethane with gradient elution. The dissociative astaxanthin elution was distilled and concentrated under temperature 35℃, vacuum 0.08 MPa condition. Its purity was 92.9%.