Abstract:

According to chicken β-defensin sequences published in GeneBank, a pair of specific primers was designed. The Gal-9 gene was amplified by using RT-PCR from the lung tissue of Xinzheng Sanhuang chicken. Then it was sequenced. The results showed that the length of the cDNA fragment obtained was 195bp, encoding 65 amino acids; BLAST analysis showed that the homology between the fragment cloned and the chicken β-defensin-Gal-9 gene sequences published in the Gene Bank was 98%. Then the sequence was contrasted with the 10 Gal-9 sequences published, at the same time, the phylogenetic tree was drawn. The results showed that the homology of them is from 91.8% to 94.4%; Phylogenetic tree analysis showed that the gene and Gal-9 gene in the same evolutionary branch. That not only provided a base for research on immunologic function of Gal-9 in the native chicken, but also laid the foundation of developing new antimcrobial product with broad spectrum.