Abstract:

In order to explore and improve the aquatic plant Bacopa monnieri with biotechnological methods, regeneration from tissue culture and Agrobacterium-mediated genetic transformation system were established with leaves of Bacopa monnieri as explants. The results showed that the optimal callus induction medium was MS+6- BA 0.5 mg /L+NAA 0.02 mg/L; differentiation medium was MS+6-BA 0.5 mg/L+NAA 0.05 mg/L; rooting medium was 1/2MS+IBA 0.1 mg/L. The best Agrobacterium tumefaciens-mediated genetic transformation conditions were as follows, explants pre-culturing for 2 day, OD600 of infecting bacilli 0.5, infection for 7 min, co-culturing for 4 day and delay screening after 4 day. The highest frequency of transformation occurred in 21.4%.