Abstract:

The rapid and efficient regeneration system was build by using Anthurium andraeanum young leaf and petiole as explants. The results indicated that the culture media which contains different components has different induction effect to different plants tissue, like leaf, petiole and adventitious bud. Further, we discovered that the most suitable leaf and petiole callus induction media were 1/2MS +1.0 mg/L 6-BA +0.2 mg/L 2,4-D and 1/2MS +1.0 mg/L 6-BA +0.1 mg/L 2,4-D, the media containing 1/2MS +2.0 mg/L KT +0.1 mg/L NAA was well to induce adventitious buds , the media containing 1/2MS+2.0 mg/L 6-BA+0.2 mg/L NAA was easy to regenerate and rejuvenation for ,and the media containing 1/2MS+0.05 mg/L NAA was easy to regenerate root for seedlings.We also found that the aneuploid chromosome variation frequency of Anthurium andraeanum callus was increased as generation times during Anthurium andraeanum callus subculture.