Abstract:

In this paper，a RNAi approach was used in down-regulated CaMBP10 gene expression to study its functions in vivo. A 273 bp fragment of CaMBP10 cDNA was cloned from Chinese cabbage “JINYU 60” and inserted into the RNAi vector PTCK303. According to the results of detections, sense and anti-sense of CaMBP10 fragments had been cloned into RNAi victor between the left and right of an intro from rice. The Chinese cabbage “JINYU 60” was transformed by Agrobacterium tumefaciens LBA4404 which harbored this anti-sense CaMBP10 gene. The result of RT-PCR analysis shows that the CaMBP10 gene expression was reduced in transgenic plants. This work would serve as a good base for following research.