Abstract:

In order to increase the propagation rate for mass-produced Ajuga lobata and provide the technical guidance for the safety of germplasm resources and effectively preserve, the tissue culture and rapid propagation of Ajuga lobata was studied. The regeneration system of Ajuga lobata was well established by leaf blade. The results summarized as fellow: With 10 min NaClO 3% it can achieve good results by using leaves as explants. MS +6-BA 1 mg/L was the optimum multiplication culture medium. It can get high proliferation rate. MS+6-BA 1mg/L+2,4-D 0.4 mg/L was the optimum callus culture medium. The callus was large and green. MS+IBA 0.4 mg/L was the optimum culture medium taking roots. The roots were flourishing and the plant was strong. Thus the regeneration system of Ajuga lobata was established and it provided a foundation for Ajuga lobata mass production.