Abstract:

Endophytic fungi play a key role in growth and anti-adversity of Pyrola. The biological characteristics of a strain of endophytic fungi R25 (Nectria sp.), which was isolated from Pyrola, were studied to provide evidence for appliance of the strain to introduction and acclimatization of Pyrola. The results showed as follows. The strain grew rapidly during the third to tenth day, followed by stable growth period after the eleventh day. The proper growth temperature of the strain was 10～25℃, and the optimal was 20℃. The optimum growth pH of the strain was 5.0～6.0, and darkness was beneficial to the growth. The strain had the ability of utilizing a wide range of carbon and nitrogen sources, and the optimum carbon and nitrogen sources were glucose and KNO3, respectively. Growing on the OMA media, the strain reached the peak capacity of generating spores.