Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (7): 46-49.

Special Issue: 小麦

• 23 • Previous Articles     Next Articles

Analysis on the Offsprings from Crossing between Wheat-Rye Substitution Lines by SSR

  

  • Received:2010-08-19 Revised:2010-09-23 Online:2011-04-05 Published:2011-04-05

Abstract:

In order to study their genetic component and use them better, seven lines, 08-4、08-5、08-6、08-7、08-8、08-9、08-12,with big ears which came from crossing between wheat-rye substitution line 5R/5A and 6R/6A were identified by SSR.The results are followed: 17 SSR primer pairs located on chromosomes of rye were used for SSR amplification.The specific DNA fragments of rye, 168bp、178bp、190bp ,can be amplified by pimer Xgwm247-6RL in the lines 08-4、08-8、08-12. These results indicated that lines ,08-4、08-8、08-12, contained rye chromatin of 6RL. Because they have big ears and multispikelets with more number of grains per ear, these lines have very important value in wheat breeding and genetics improvement .