Abstract:

Soluble starch synthaseⅡ (SSⅡ) is the main character of the enzymes that catalyse amylopectin synthesis, the exploration of its three homoeologous genes’ (SSⅡ-A, SSⅡ-B and SSⅡ-D) contribution to amylopectin synthesis is very important to the genetic manipulation of starch synthesis and the improvement of starch quality in wheat. Here we cloned partial sequence (539 bp) of wheat SSⅡ-A cDNA using RT-PCR technology, which completely aligns to AB201445.1 (Triticum aestivum wSSII-A gene for starch synthase II-A, complete cds). The hairpin structure of the sequence was constructed based on H plasmid, and then it was ligated to the downstream of HMW-GS 1Dx5 promoter in pBAC47P. Therefore, the specific and effective RNAi expression vector was formed, which would lay a foundation to further explore SSⅡ-A’s contribution to amylopectin synthesis.