Abstract:

Five isozymes (EST, CAT, POD, MDH, LDH) in six tissues (liver, heart, spleen, kidney, muscle, eye) of Hephaestus fuliginosus were studied by using vertical polyacrylamide gel gradient electrophoresis. Zymograms and loci of these 5 isozymes were analyzed. The results showed that these five isozymes presented tissue specificity to some degrees. Two sites were detected with EST and each site was encoded by 2 loci. Only one site was detected with CAT which included 2 loci. The zymogram of POD was relatively complicated, includeding 5 sites and 4 of which had alleles. Two types of MDH (m-MDH and s-MDH) were found in six tissues: s-MDH only showed one dimer; m-MDH had 2 bands in liver and 1 bands in other tissues. LDH was detected in six tissues and Ldh-5 was only found in heart.