Abstract:

Flavonol synthase gene (FLS) is very important in the flavonoids pathway, and it determines the ginkgo flavonoids accumulation directly. The study of the gene expression and regulation is important to improve the ginkgo flavonoids content in vivo. However, how the gene is regulated at transcriptional level is still unclear. To clarify the potential mechanism, a FLS promoter from ginkgo biloba has been cloned and sequenced. The results of bioinformatics analysis showed that three MYB binding sites and nine sites related to light signal existed in the FLS promoter, which had provided a molecular basis that could illustrate the transcriptional regulation mechanism of FLS gene light signal response and the co-regulated relation between FLS and CHI.