Abstract:

Abstract: Objective: To establish a highly efficient, cost-effective and handily detection method for the diagnosis of porcine epidemic diarrhea; Method: Based on the well known working principle of Gold-Immunochromatography Assay (GICA), a batch of in-house GICA test strips were made by assembling the following elements, which were included the protein SPA labeled with HAuCl4 as indicating reagent as well as the recombinant PEDV M protein and multiple anti-SPA serum coated onto the NC membrane as detective line and control line respectively. Results: Compared with the reference ELISA method, the established GICA shared the similar test sensitivity. Especially, the clinical test agreement rate of both methods reached 96%, which was calculated by the sum percent of positive (65) and negative (7) samples among total 75 clinical samples. Conclusion: This GICA method provided the most rapid and convenient means for detection of PEDV infection in field.