Abstract: The effect of different calcium fertilizers on quality and Ca2+-ATPase activity was conducted with spraying amino acid calcium, calcium Cuikang(Stopit), calcium 2000 and JuJin calcium(Fulvic acid calcium) in red Fuji apple. The results showed that calcium had increased the single fruit weight, L/D, Vc content, SSC content and anthocyanidin content, while had decreased the chlorophyll content and titratable acid. In all tested calcium fertilizers, the Jujin calcium was the best, then the amino acid calcium, Cuikang calcium, calcium 2000 respectively. Ca2+-ATPase activity in calcium-treated fruits are significantly higher than that in control, and the change of Ca2+-ATPase activity had the similar tendency with that of fruit quality o apple.