Abstract:

A full-length cDNA sequences of Cu/Zn-SOD was cloned from Myrica rubra leaves by using the RT-PCR technology, and the expression levels of this gene in different tissues were found through Semi-Quantitative RT-PCR method. The full-length is 461 bp encoding a 152 amino acids Cu/Zn-SOD ortholog. The sequences contained the Cu/Zn-SOD family signature sequence and had the homology more than 77% comparing with other Cu/Zn-SOD from GenBank. This gene was named as MrSOD1 (GenBank accession No. GQ404510). The expression levels of MrSOD1 in different tissues were fruit>leaf>flower >shoot. The study provided a way for deep research on the expression and regulation of MrSOD1, and the molecular mechanism of anti-oxidative effect in Myrica rubra.