Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2011, Vol. 27 ›› Issue (4): 96-101.

Special Issue: 生物技术 园艺

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Construction and Preliminary Analyses of SSH cDNA Libraries of Anji Baicha (Camellia sinensis)

  

  • Received:2010-10-11 Revised:2010-11-30 Online:2011-02-10 Published:2011-02-10

Abstract:

To separate the differentially expressed genes between white and green period leaf of ‘Anji Baicha’, a subtracted library was constructed by the suppression subtractive hybridization (SSH) method. By sequencing the 80 recombinant clones and through BlastX analysis, 30 showed no homologous, 8 had short sequence and also showed poor homologous, for the other 42 fragments in 80 recombinant clones. They showed more or less homologous to all proteins in the database. Three fragments might have correlation with high content of amino acid in ‘Anji Baicha’.