Abstract:

A series of experiments were made on how to filtrating tomato axenic seedling-age, hormone constitution on shooting and rooting medium in order to obtain the best way on regenerative system of tomato. ‘Zheza 905’, ‘Shengya’ and ‘Fudan’ were used as the materials in the study. Tomato stem and cotyledon were cultured to study on the callus rate, induction rate and rooting rate. The results showed that the best seedling-age were 4 days. The best regeneration medium of tomato cotyledon explants included MSB5, 3% sucrose, 0.6% agar, BA 2.0 mg/L, NAA 0.1 mg/L with pH5.8. The cotyledon callus rates of ‘Zheza 905’, ‘Shengya’ and ‘Fudan’ were 97.00%, 91.00% and 86.00%, respectively. The induction rate were 64.67%, 59% and 53%, respectively .While the medium for stem explants includes MSB5, 3.0% sucrose, 0.6% agar, 2.0 mg/L BA, 0.2 mg/L NAA with pH5.8. The stem callus rate of‘Zheza 905’, ‘Shengya’ and ‘Fudan’ were 81.67%, 76.00% and 82.67%, respectively. The induction rates were 41.00%, 48.67% and 39.67%, respectively. The rooting medium included 1/2MS, IBA0.5 mg/L, NAA 0.1 mg/L. Regeneration frequency from cotyledon was much higher than that of stems.