Abstract:

To study the optimal extraction conditions of flavonoids from peanut hulls by the method of microwave extraction, on the yield of flavonoids, a central composite design (CCD) experiment was conducted and the response surface methodology (RSM) was employed to investigate the influence of four main factors, including microwave output power, microwave extraction irradiation time, ratios of solution to material and alcohol concentration. A quadratic polynomial regression equation of the forecasting model was set up. Regression coefficient and variance analysis showed the regression model fited the actual process well. The optimal conditions of microwave extraction were concluded as follows: ratios of solvent to material 1 g:30 mL, alcohol concentration 78%, microwave output power 460 w, microwave extraction irradiation time 120 s. Based on these conditions, the yield was 2.918 g/100 g. By colour reaction and paper chromatography, the products were flavonoids.