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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (23): 27-30.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

奶牛HSP 70表达量与温湿指数的相关性研究

甘乾福   

  • 收稿日期:2009-08-18 修回日期:2009-08-26 出版日期:2009-12-05 发布日期:2009-12-05
  • 基金资助:
    国家科技支撑计划（奶业专项）;福建省科技重大专项

Study on the relationship between heat stress protein 70 of dairy cow in blood lymphocytes and temperature humidity index

  • Received:2009-08-18 Revised:2009-08-26 Online:2009-12-05 Published:2009-12-05

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摘要： 利用免疫学和分子生物学手段研究荷斯坦牛和娟-荷F1牛，在环境温湿指数(THI)影响下，血液淋巴细胞热应激蛋白70(HSP 70)的表达情况，旨在探索HSP 70表达量与THI相关性以及不同品种差异性。结果表明：(1)荷斯坦成乳牛在热应激和非热应激条件下均可表达HSP 70，严重热应激期(THI 93.5±2.1)HSP 70表达量分别是中度热应激期(THI 76.7±2.6)1.9倍(P<0.01)和非热应激期(THI 56.5±1.7)6.9倍(P<0.01)，中度热应激期HSP 70表达量是非热应激期3.6倍(P<0.05)，HSP 70表达量与THI呈强度正相关(R=0.91)(P<0.01)，THI每上升1个单位，HSP 70表达量增加2172 OD/mm2；(2)在严重热应(THI 95.3)条件下，娟-荷F1成乳牛HSP 70表达量极显著高于荷斯坦牛(P<0.01)。 关键词：奶牛；热应激蛋白；温湿指数；相关性

关键词: 成功度评价法, 成功度评价法, 绩效评价, 层次分析法, 农业灌溉项目

Abstract: In order to explore the expression of heat stress protein 70 ( HSP 70), the relationship between HSP 70 and temperature humidity index (THI) and the different breeds expression of HSP 70 in heat stress in blood lymphocytes. We used immunology and molecular biology methods to study the influence of environmental factors THI on Holstein and Jersey×Holstein F1 dairy cows. The result show that: (1) during heat and no stress period, the Holstein dairy cows can express HSP 70. Comparing serious heat stress period (THI 93.5±2.1) with moderate heat stress period (THI 76.7±2.6) and no heat stress period(THI 56.5±1.7), the expression of HSP 70 was 1.9 times (P<0.01) than that of moderate heat stress period and 6.9 times (P<0.01) than that of no heat stress period. The expression of HSP 70 in moderate heat stress period was 3.6 times (P<0.05) than that of no heat stress period, the relationship between the expression of HSP 70 and THI were positive(P<0.01)(R=0.91), THI rise 1 unit, the expression of HSP 70 increase 2172 OD/mm2. (2) The expression of HSP 70 in Jersey×Holstein F1 dairy cows were higher than that of Holstein dairy cows (P<0.01) during serious heat stress period (THI 95.3).