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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (23): 31-33.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

鸡粪便中氧氟沙星残留检测微生物法的建立

胡晓苗   

  • 收稿日期:2009-07-15 出版日期:2009-12-05 发布日期:2009-12-05
  • 基金资助:
    国家科技富民强县专项行动计划:（铜陵县）渔牧结合高效循环养殖技术集成示范与推广和现代农业产业技术体系建设专项资金、公益性行业蛋鸡科研专项基金项目:优质土鸡蛋生产关键技术研究与示范

Determination of ofloxacin residues in faeces of chicken by Microbiological Method

  • Received:2009-07-15 Online:2009-12-05 Published:2009-12-05

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摘要： 以金黄色葡萄球菌为工作菌, 用杯碟法测定氧氟沙星（OFL）在畜禽粪便中的残留。结果表明：鸡粪便中氧氟沙星的最低检测限为0.156μg/g,标准曲线的工作范围为0.156~5.00μg/g，工作曲线的日内变异系数在2.4%~3.3%、日间变异系数为3.7%~5.2%，回收率分别为79.49%~87.92%。因此,建立的方法可以满足鸡粪尿混合物中氧氟沙星的残留检测要求。

关键词: 黄瓜, 黄瓜, 病害, 遗传规律, 分子标记

Abstract: Ofloxacin residues in were determined using cylinder plate method, taking Micrococcus luteus 26003 as the test microorganism. Results showed that the detection limit of ofloxacin in faeces was 0.156μg/g, and the calibration curve was linear within the range of 0.156~5.00μg/g , the coefficients of variation for the microbiological method were 2.4%~3.3% in intra-assay and 3.7%~5.2% in inter-assay, the recoveries in faeces of chicken ranged from 79.49% to 87.92%.