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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (7): 338-342.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

鸡源益生菌JM-11菌株的胃肠道耐受及其抗腹泻的研究

郝庆红 郭云霞 吴国江 王世英 朱宝成   

  • 收稿日期:2010-10-18 修回日期:2010-12-15 出版日期:2011-04-05 发布日期:2011-04-05

The tolerance in gastrointestinal tract environment and anti-diarrhea of strain JM-11 from chicken against pathogenic bacteria

  • Received:2010-10-18 Revised:2010-12-15 Online:2011-04-05 Published:2011-04-05

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摘要：

本试验从健康的鸡盲肠内容物中筛选出具有较高抑菌活性的病源拮抗菌JM-11菌株，经形态、生理生化及16S rDNA序列鉴定此菌为枯草芽孢杆菌（Bacillus subtilis subsp. subtilis）。经耐受胃肠道环境及胆盐试验，结果表明，JM-11菌株能耐受0.3%的胆盐环境，在pH为3.0的人工胃液中能够很好的存活。利用抗生素打乱雏鸡肠道菌群后，饲喂致病性大肠杆菌来制作腹泻模型。经益生菌JM-11对腹泻雏鸡的治疗试验结果显示，JM-11能显著降低腹泻雏鸡粪便中的大肠杆菌的数量（P<0.01），增加乳酸菌的数量(P<0.01)，提高雏鸡免疫器官指数(P<0.05)，对雏鸡具有安全无毒的作用。

关键词: 蓖麻, 蓖麻, 丰产性, 稳定性, 杂交品种, 淄蓖麻7号, 淄蓖麻5号

Abstract:

In this study，strain JM-11 with antagonistic activity against E. coli was isolated from the cecum of healthy chicken. As the morphology characteristics and physiological and biochemical characteristics of the strain JM-11 was finally identified as Bacillus subtilis subsp. subtilis. The test result of the tolerance in gastrointestinal tract and Bile salts were showed as follows: the strain JM-11 can survive in the simulated natural (bile salts content 0.3%, pH3.0 artificial gastric juice)environment. The model of diarrhea was produced by using antibiotics to break flora balance of intestinal tract, then pathogenicity E.coli was fed. The result of curing diarrhea of chicken by strain JM-11 was showed as follows: the quantity of E.coli was remarkably decreased (P<0.01), Lactobacilli activities significantly increased (P<0.01), immune organ index of chicken was significantly increased(P<0.05).