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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (3): 272-276.doi: 10.11924/j.issn.1000-6850.2011-2270

所属专题： 马铃薯

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不同样品分散温度对双波长比色法测定木薯直、支链淀粉比率及总淀粉含量的影响

黄惠芳 罗燕春 候学文 符海天 彭靖茹 黎萍 黄强   

  • 收稿日期:2011-08-04 修回日期:2011-11-04 出版日期:2012-01-25 发布日期:2012-01-25
  • 基金资助:

    高淀粉产量木薯分子育种技术研究

The Affection that Different Temperature of Sample Dispersion to Ratio of Amylose and Amylopectin, Total Starch Content with Double Wavelength Spectrophotometric on Cassava

  • Received:2011-08-04 Revised:2011-11-04 Online:2012-01-25 Published:2012-01-25

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摘要：

为了研究木薯直、支链淀粉比率及总淀粉含量的测定方法，通过采用双波长比色法，研究不同样品分散温度对木薯淀粉直、支链淀粉比率及总淀粉含量的影响。结果表明：在试验温度70~100℃范围内，85℃时直链淀粉百分比率最低，相对应地，85℃时支链淀粉的百分比率最高。样品分散温度对鲜薯中总淀粉含量测定值有影响，其测定值在80℃时最高。由此可知，不同样品分散温度对木薯淀粉直、支链淀粉比率及总淀粉含量测定值有影响。

关键词: 木薯, 木薯, 干旱胁迫, 土壤相对含水量, 光合作用, 光响应

Abstract:

In order to study determination of the ratio of amylose and amylopectin, total starch content for cassava roots. By using double wavelength spectrophotometric, study different temperature of sample dispersion affect the percentage rate of amylase and amylopectin, total starch content on cassava. The results showed that: within the temperature of 70℃ to 100℃, when the temperature was 85℃, the percentage of amylose was the relative minimum. Correspond to, when it was 85℃, the percentage of amylopectin was the highest. Different temperature of sample dispersion affect total starch content in fresh cassava roots, when the temperature was 80℃, the determination was the maximum. The temperature of sample dispersion affected the determination results of the ratio of amylose, amylopectin, total starch content.

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