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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (10): 219-224.doi: 10.11924/j.issn.1000-6850.2011-2920

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

宽筋藤组织培养的初步研究

陈加利 杨琪 林海叶 于旭东 吴繁花   

  • 收稿日期:2011-10-10 修回日期:2012-01-13 出版日期:2012-04-05 发布日期:2012-04-05

The Preliminary Study on Tissue Culture of Tinospora sinensis

  • Received:2011-10-10 Revised:2012-01-13 Online:2012-04-05 Published:2012-04-05

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摘要：

为了研究宽筋藤快繁技术，以宽筋藤健壮无病虫害的嫩茎和嫩叶作为试验材料，研究其表面消毒方法和在添加不同浓度激素的培养基上培养，对宽筋藤进行组织培养研究。结果表明：茎段表面采用75%酒精消毒30 s，用0.1%升汞浸泡5 min，无菌水漂洗2次后，再用0.1%升汞浸泡5 min，无菌水漂洗4次的效果最佳，存活率为66.7%；而叶片直接用升汞处理12 min的消毒效果较好，存活率为53.3%；适宜侧芽诱导和增殖的培养基为MS+6-BA 0.05 mg/L+GA3 0.04 mg/L+NAA 0.02 mg/L，30天的增殖系数为3；诱导愈伤组织以培养基MS+2,4-D 1.5 mg/L+BA 0.5 mg/L的效果较好，对嫩茎的诱导率可达83.3%，对嫩叶的诱导率可达76.7%，但所诱导的愈伤组织分化能力低，均不能分化不定芽；适宜生根的培养基为MS+NAA 0.1 mg/L，生根率可达到100%，且移栽成活率96%。

关键词: 连作障碍, 连作障碍

Abstract:

In order to study multiplication culture of Tinospora sinensis, using the robust and pest-free young spears and leaves of the Tinospora sinensis as the research material, this article was to examine the disinfection methods and to study the tissue culture of the Tinospora sinensis through cultivating them in the hormone mediums of different concentrations. According to the results, if the spear surface was disinfected with the alcohol of 70% for 30 seconds, then soaked in the mercury of 0.1% for 5 minutes, rinsed with the sterile water twice, then soaked in the mercury of 0.1% for 5 minutes, rinsed with the sterile water 4 times, the outcome would be the best that the survival rate was 66.7%; while the leaves can be disinfected effectively with mercury for 12 minutes as the survival rate was 53.3%; the medium suitable for the bud induction and proliferation was MS+6-BA 0.05 mg/L+GA3 0.04 mg/L+NAA 0.02 mg/L, the multiplication coefficient for 30 days was 3; the medium suitable for callus induction was MS+2,4-D 1.5 mg/L+BA 0.5 mg/L, the spear induction rate can be as high as 83.3%, and the leave induction rate can be 76.7%, but the inducted callus has low differentiation, unable to differentiate adventitious buds; the medium suitable for root taking was MS+ NAA 0.1 mg/L, the root taking rate can be 100% and the transplant survival rate can be 96%.