关键词: PRRSV-FJ04A, PRRSV-FJ04A, 病理学, 分布

Abstract: The authors used‘Heinong 48’ (high- protein type) soybean as material, inoculated the Fueneliformis mosseae into soils surrounding the root system, and conducted continuous cropping by spatial method instead of time method. Then the F. mosseae infection on soybean roots was detected by supplying the acid magenta to calculate the incidence of root rot according to the following classification standard. Also, chemical titration and colorimetry were used to detect the changes of enzyme activity in treated soybean root system as well as rhizosphere soil. In the process of experiment, we selected soil of different continuous cropping years (1, 2 and 4 years) for pot trials, and set the non-inoculation of F. mosseaeas a control group (C) while made the inoculation of F. mosseae as a treatment group (T). The results showed that, as continuous cropping years increased, the enzyme activities of catalase (CAT), polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) in soybean roots increased, but the SOD enzyme activity showed a downward trend. In addition, with the growth of soybean, soil enzyme activity decreased gradually, in which soil urease (URE), CAT, sucrose (SUC), phosphatase (PHO) and PPO activity decreased first and then increased, and the activity of cellulase (CEL) gradually decreased. In conclusion, the defense enzyme activity and soil enzyme activity of soybean roots after F. mosseae inoculation were significantly higher than that of the control group, which indicated that F. mosseae could alleviate continuous cropping obstacle and improve disease resistance of plant.