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中国农学通报 ›› 2021, Vol. 37 ›› Issue (24): 116-123.doi: 10.11924/j.issn.1000-6850.casb2020-0474

• 生物科学 • 上一篇    下一篇

基于16S rDNA扩增子测序分析灵芝连作覆土细菌群落的变化

袁源1,2(), 李琳1,2, 黄海辰1, 刘国辉3, 谢福泉4, 傅俊生1,2(), 吴小平1,2()   

  1. 1福建农林大学生命科学学院,福州 350002
    2福建农林大学菌物研究中心,福州 350002
    3仙芝科技(福建)股份有限公司,福建南平 353400
    4福建省食用菌技术推广总站,福州 350003
  • 收稿日期:2020-09-18 修回日期:2020-12-16 出版日期:2021-08-25 发布日期:2021-08-27
  • 通讯作者: 傅俊生,吴小平
  • 作者简介:袁源,女,1995年出生,云南昭通人,硕士,主要从事食药用真菌活性成分的研究。通信地址:350002 福建省福州市仓山区上下店路15号 福建农林大学生命科学学院,Tel:0591-18860174918,E-mail: 424515867@qq.com
  • 基金资助:
    国家重点研发计划项目“闽产高品质道地中药材灵芝、太子参规范化种植及精准扶贫示范研究”(2019YFC1710500);2019年福建省现代农业食用菌产业技术体系建设项目“现代食用菌产业技术体系岗位专家工作站–秀珍菇、灵芝岗位”(闽农综[2019]144号)

Analysis of Bacterial Community in Ganoderma lingzhi Continuous Cropping Soil Based on 16S rDNA Amplicon Sequencing

Yuan Yuan1,2(), Li Lin1,2, Huang Haichen1, Liu Guohui3, Xie Fuquan4, Fu Junsheng1,2(), Wu Xiaoping1,2()   

  1. 1College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002
    2Mycological Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002
    3GanoHerb Technology (Fujian) Corporation, Nanping Fujian 353400
    4Fujian Edible Fungi Technical Popularized Station, Fuzhou 350003
  • Received:2020-09-18 Revised:2020-12-16 Online:2021-08-25 Published:2021-08-27
  • Contact: Fu Junsheng,Wu Xiaoping

摘要:

揭示不同连作年限灵芝覆土中细菌群落的变化情况,为灵芝连作障碍机制的研究提供理论依据。以邻近沙壤土(GL0)为对照,采用Illumina MiSeq平台对灵芝栽培1年(GL1)、连作1年(GL2)、2年(GL3)和3年(GL4)的覆土进行扩增子测序。多样性分析表明,连作会加大灵芝覆土中细菌群落的物种组成差异,且细菌群落的丰富度和多样性均呈现先增加后减少的趋势。在门水平上,变形菌门和放线菌门的相对丰度随着连作年限的增加而显著性增加;绿弯菌门的相对丰度逐年递减。在属水平上,鞘氨醇单胞菌属、Anaeromyxobacter、慢生根瘤菌属、脱氯菌属和Ktedonobacter等在灵芝覆土中的相对丰度逐年递减,溶杆菌属、Gp6和Gp16的相对丰度显著提高。灵芝连作障碍可能与覆土中鞘氨醇单胞菌属、Anaeromyxobacter、慢生根瘤菌属、脱氯菌属等有益细菌相对丰度逐年降低有关。

关键词: 灵芝, 细菌群落, 细菌多样性, 扩增子测序, 连作障碍

Abstract:

This study aims at revealing the changes of bacterial community in the covering soil of Ganoderma lingzhi with different continuous cropping years, and providing a theoretical basis for the study of continuous cropping obstacle mechanism of Ganoderma lingzhi. Taking the adjacent sandy soil (GL0) as the control, Illumina MiSeq platform was used for the amplicon sequencing of the covering soil of Ganoderma lingzhi cultivated for 1 year (GL1), and continuous cropped for 1 year (GL2), 2 years (GL3), and 3 years (GL4). The diversity analysis showed that continuous cropping increased the difference in species composition of bacterial communities in the covering soil of Ganoderma lingzhi, and the abundance and diversity of bacterial communities showed a tendency of increasing first and then decreasing. At the level of phylum, the relative abundance of Proteobacteria and Actinomycetes increased significantly with the increase of continuous cropping years, while that of Chloroflex decreased. At the genus level, the relative abundance of Sphingomonas, Anaeromyxobacter, Bradyrhizobium, Dehalococcoides and Ktedonobacter in the covering soil of Ganoderma lingzhi reduced year by year; while that of Lysobacter, Gp6 and Gp16 dramatically increased. Continuous cropping obstacle of Ganoderma lingzhi may be related to the year-by-year decrease of the relative abundance of beneficial bacteria such as Sphingomonas, Anaeromyxobacter, Bradyrhizobium and Dehalococcoides and so on in covering soil.

Key words: Ganoderma lingzhi, bacterial communities, bacterial diversity, amplicon sequencing, continuous cropping obstacle

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