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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (10): 142-145.doi: 10.11924/j.issn.1000-6850.2011-3190

所属专题： 食用菌； 食用菌

• 林学 园艺 园林 • 上一篇    下一篇

11个毛木耳主栽菌株的鉴别研究

殷贝贝 刘宇 赵爽 郑翠芳 王守现 许峰 耿小丽 孟莉莉   

  • 收稿日期:2011-11-01 修回日期:2011-11-28 出版日期:2012-04-05 发布日期:2012-04-05

The Identification Research of 11 Auricularia polytricha Mont. Sacc Strains Cultivated

  • Received:2011-11-01 Revised:2011-11-28 Online:2012-04-05 Published:2012-04-05

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摘要：

为了对实验室保藏的11个毛木耳品种进行菌株鉴别，以期减少实际生产中的同种异名现象，为指导生产提供依据。利用拮抗试验和酯酶同工酶鉴定法对不同的毛木耳栽培菌株进行鉴别。结果表明，两种方法得到了一致的结论。11个菌株分为六类，第一类包括毛木耳3号等四个菌株；第二类包括白背120等3个菌株；其他菌株各分属不同类别。通过研究认为在食用菌菌种的鉴别中，应用拮抗试验对供试菌株进行初步筛选是必要的，可以减少工作量和盲目性，为进一步的研究提供依据。同时酯酶同工酶鉴定法从分子方面验证了菌株类别，为遗传学研究和育种提供了依据。

关键词: 检测, 检测

Abstract:

The aim of this research was to distinguish 11 cultivated strains of Auricularia polytricha Mont. They were analyzed by antagonistic test and Esterase Isoenzyme, The results showed that the two methods got the same conclusions, and 11 strains could be divided into six groups: the first group including 4 strains of Auricularia polytricha Mont. No.3 etc. the second group including 3 strains of white back 120 etc. The others belonged to different groups. Therefore, in order to reduce the workload and blindness, and to provide a basis for further research, it was necessary to utilize antagonistic test for a preliminary screening on tested strains in the identification of edible fungi strains. Esterase Isoenzyme was also effective for the classification of strain, Its results could be comprehensively analyzed for providing the basis in genetics research and mushroom breeding.