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中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (4): 98-104.doi: 10.11924/j.issn.1000-6850.casb20200300201

所属专题： 生物技术； 小麦； 农业生态

• 植物保护·农药 • 上一篇    下一篇

Fhb1基因改良‘周麦’品种赤霉病抗性研究

李楠楠(), 李顺成, 韩玉林(), 邹少奎, 杜晓宇, 杨光宇, 王丽娜, 张倩, 吕永军   

  1. 周口市农业科学院/河南省小麦种质改良工程技术研究中心/河南省作物分子育种与生物反应器重点实验室,河南周口 466001
  • 收稿日期:2020-03-13 修回日期:2020-04-27 出版日期:2021-02-05 发布日期:2021-01-25
  • 通讯作者: 韩玉林
  • 作者简介:李楠楠,男,1988年出生,河南周口人,助理研究员,硕士,从事小麦遗传育种工作。通信地址：466001 河南省周口市川汇区建设东路4号,E-mail： 18638099528@163.com。
  • 基金资助:
    国家重点研发计划“小麦分子设计育种”(2016YFD0101802);国家重点研发计划“超高产育种新材料创制与新品种选育”(2017YFD0100700);国家小麦产业技术体系(CARS-03-02-33);河南省小麦产业技术体系(Z2010-01-11);河南省重大科技专项“优质专用小麦新品种与绿色增效生产技术示范与推广”(181100110200)

Research of Resistance in ‘Zhoumai’ Wheat Cultivars to Fusarium Head Blight (FHB) with Fhb1 Gene

Li Nannan(), Li Shuncheng, Han Yulin(), Zou Shaokui, Du Xiaoyu, Yang Guangyu, Wang Lina, Zhang Qian, Lv Yongjun   

  1. Zhoukou Academy of Agricultural Sciences/ Wheat Germplasm Improvement Engineering Research Center of Henan Province/ Key Laboratory of Crop Molecular Breeding and Bioreactor of Henan Province, Zhoukou Henan 466001
  • Received:2020-03-13 Revised:2020-04-27 Online:2021-02-05 Published:2021-01-25
  • Contact: Han Yulin

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摘要：

为加快‘周麦’品种赤霉病抗性改良,利用当地主栽‘周麦’品种（系）‘周麦22号’、‘周麦32号’、‘周11550’为母本,‘宁麦9号’、‘生选6号’、‘扬麦21号’等长江中下游地区抗赤霉病材料为父本配制一系列杂交组合,经过选择获得621份F3~F6后代材料。在田间选用来源于江苏和河南两地不同的赤霉病菌株,通过单花滴注法接种小穗进行赤霉病抗性鉴定,同时利用抗赤霉病主效基因Fhb1紧密连锁的诊断性标记His-InDel对后代材料进行分子检测。结果表明,江苏的赤霉病菌株接种后代材料田间鉴定为高抗和中抗的占总数的23.9%,而河南的赤霉病菌株感染后代材料田间鉴定为高抗和中抗占比为35.1%,说明后代材料的赤霉病抗性比感病亲本有明显的改良,而且材料对江苏的赤霉菌菌株的抗性比河南的赤霉菌菌株的抗性低。分子检测结果显示,携带Fhb1和不携带Fhb1的材料之间赤霉病抗性差异极显著。这表明利用Fhb1基因分子标记辅助选择技术可用于改良‘周麦’品种的赤霉病抗性。

关键词: 小麦, 赤霉病, Fhb1基因, 分子检测, 田间鉴定

Abstract:

To accelerate the improvement of wheat resistance to fusarium head blight (FHB) in ‘Zhoumai’ wheat, we used the local main wheat varieties (lines) ‘Zhoumai 22’, ‘Zhoumai 32’ and ‘Zhou11550’ as the female parents and the ‘Ningmai 9’, ‘Shengxuan 6’, ‘Yangmai 21’, which are the FHB resistant materials in the middle and lower reaches of the Yangtze River, as male parents to make a series of hybrid combinations. Then we got 621 selected materials in F3-F6 offspring. In the field, we used different FHB strains from Jiangsu and Henan to identify the resistance to FHB through inoculating spores into spikelets by single flower drip method. Meanwhile, the molecular detection of the progenitor materials was carried out by using the closely linked diagnostic marker His-InDel of the main gene Fhb1. Field inoculation results showed that 23.9% of the total offspring from Jiangsu had high or moderate resistance to FHB, while this rate was 35.1% in the offspring of Henan. The resistance of the offspring materials was significantly improved compared with their parents, which were susceptible to FHB. And the resistance of the materials to strains from Jiangsu was lower than that from Henan. Molecular detection results showed that the resistance to FHB had a significant difference in materials containing Fhb1 or not, indicating that Fhb1 gene marker assisted selection could be used to improve the resistance of wheat cultivars to FHB efficiently.

Key words: wheat, fusarium head blight (FHB), Fhb1 gene, molecular identification, field resistance

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