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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (10): 95-102.doi: 10.11924/j.issn.1000-6850.2011-3601

所属专题： 烟草种植与生产

• 林学 园艺 园林 • 上一篇    下一篇

利用SSR荧光标记技术分析烟草种质的遗传多样性

何其芳 李荣华 郭培国 宁正祥 邱妙文 赵伟才 夏岩石 白盼   

  • 收稿日期:2011-12-02 修回日期:2012-01-13 出版日期:2012-04-05 发布日期:2012-04-05
  • 基金资助:

    国家自然科学基金;国家烟草总公司面上项目;广东省烟草公司科技计划

Genetic Diversity Analysis for Tobacco Germplasm by Using Fluorescent SSR Technique

  • Received:2011-12-02 Revised:2012-01-13 Online:2012-04-05 Published:2012-04-05

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912

摘要：

针对中国烟草栽培品种遗传基础狭窄的情况，分析烟草种资资源的遗传多样性将可为烟草新品种选育、引种和资源保护提供重要信息。据此，利用20个SSR引物组合，采用荧光SSR标记法对来自不同国家和地区的50个烟草种质材料的遗传多样性进行分析。研究结果显示，20对SSR引物在50份烟草种质中检测到81个等位变异，平均每个SSR为4.1，平均多态性位点比率为68.4%；其中引物PT20457的鉴别能力最高，它的扩增多态位点数为6个，多态位点比率为100%，PIC值为0.944。研究的UPGMA聚类分析结果在遗传相似系数约为0.65处将50份烟草种质明显分成了3个组群，组群Ⅰ中只有1个广东晒烟，组群Ⅱ包括2个白肋烟，组群Ⅲ包括来自4个国家的47个品种。主坐标分析将所有种质分成了4个组群8个亚类。2种分析方法均较好地揭示了烟草属种间或栽培种品种类型间的遗传多样性与亲缘关系，可为烟草遗传育种和遗传连锁图谱构建的杂交亲本选择提供科学依据。

关键词: 蛋鸡, 蛋鸡, 产业升级, 优势, 劣势, 机会, 威胁

Abstract:

According to the situation of narrow genetic background in Chinese tobacco cultivars, the genetic diversity analysis of tobacco germplasm could provide important information for breeding and introducing cultivars, and germplasm resource protection. In this study, genetic diversity analysis for 50 tobacco cultivars collected from different countries/regions were performed by using fluorescence SSR technique with 20 SSR primer combinations, a total of 81 allelic variations among the collected tobacco germplasm were detected with an average of 4.1 alleles per locus of each SSR. The result showed that the SSR combination of PT20457 possessed the highest values of polymorphism information content (PIC) (0.944) and could be effective locus for identifying DNA variation among these tobacco cultivars. Based on the polymorphic data, fifty tobacco cultivars were classified into 3 groups by UPGMA method. Group I contains Yunluo-01 which was sun-cured tobacco and derived from Guangdong, group II contained Atnarello Maoliang and Brazil Shailiang which were burley, group III contains 47 tobacco cultivars which were derived from 4 different countries. By using principle coordinates analysis, these tobacco varieties were clustered into 4 groups and 8 subgroups. Both cluster analysis methods markers revealed the genetic diversity and genetic relationships among tobacco varieties, and could provide a scientific basis for genetic research and tobacco breeding.