欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (11): 93-96.doi: 10.11924/j.issn.1000-6850.2012-0310

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

庄河大骨鸡IGF-Ⅰ基因多样性与产肉性能关联的分析研究

王洪才 袁晓春 宋天玉   

  • 收稿日期:2012-02-07 修回日期:2012-03-01 出版日期:2012-04-15 发布日期:2012-04-15

Correlation Analysis between Polymorphism of IGF-I Gene and Meat Performance in Zhuanghe Dagu Chicken

  • Received:2012-02-07 Revised:2012-03-01 Online:2012-04-15 Published:2012-04-15

PDF (PC)

780

摘要：

为了探究庄河大骨鸡IGF-Ⅰ基因的多样性与产肉性能相关的遗传标记。以庄河大骨鸡为试验材料，利用PCR-RFLP分子遗传标记技术分析了IGF-Ⅰ基因5′非编码区域的多样性与产肉性能的关系。结果表明：该基因存在AA、AB、BB 3种基因型，基因型的频率分别为0.445、0.300、0.255，A基因和B基因频率分为0.595和0.405；BB型个体的活体重、屠体重、半净膛重、全净膛重、腿肌重、平均日增重和料肉比分别显著的高于AA型和AB型(P<0.05)，其他性状差异不显著(P>0.05)；AB型和AA型各性状间不差异(P>0.05)。IGF-Ⅰ基因的BB型对鸡的产肉性能有显著影响，但是，其是否可以作为庄河大骨鸡产肉性能的重要遗传标记还需做进一步研究。

关键词: 山地, 山地

Abstract:

The objective of this study was to confirm the polymorphism of GHR gene in Zhuanghe Dagu Chicken, and its genetic markers affecting meat performance traits. In this experiment, Zhuanghe Dagu Chickens were used to analyze the correlation between polymorphism of 5’ non-coding region of IGF-I gene by PCR-RFLP. The results showed that IGF-I was consisted of three genotypes (AA, AB and BB) and frequencies of the three genotypes were 0.445, 0.300 and 0.255 respectively. Frequencies of gene A and gene B were 0.595 and 0.405 respectively. Live weight, carcass weight, semi-eviscerated weight, eviscerated weight, leg muscle weight, average daily gain and ratio of feed and meat of chickens with BB genotype were significantly higher than those of AA and AB genotype (P<0.05), while no significant difference were found in other traits (P>0.05). In conclusion, BB genotype of IGF-I gene could significantly improved the meat performance of Zhuanghe Dagu Chickens, although further studies were required to determine its possibility as a gene marker for the meat performance traits of Zhuanghe Dagu Chicken.