关键词: 杨树, 杨树, 中林46, 溃疡病, 发病指数, 淀粉

Abstract: Using Mutator (Mu) mutation groups, separates and validates the Mu factor mediated PPR (Pentatricopeptide Repeats, PPR) mutation site. Containing active Mu transposons as male parent hybrid with maize inbred lines Z31, after repeated backcross with Z31 and selfing, obtain BC3F2 as material. Using Mu-AFLP method to separate Mu insertion flanking sequences of the PPR sites, and verify the authenticity of the insertion sites. Using Mu-AFLP method, 14 Mu factor insertion flanking sequences have been cloned. Removing 2 redundant sequences and 8 repeat sequences, the remained 4 target sequences were the object for further study. Gene genetic analysis verified 2 flanking sequences insertion for real. The function analysis, in which 1 is PPR mutation site and Mu factor inserted into the gene 5' UTR 121st bp and 122nd bp between base pairs. The genomic localization and functional analysis shows that Mu insertion site of target sequence located at chromosome 6, and belonged to PPR gene family. This gene could be encoded PPR protein, so we hypothesized that this Mu insertion site influences corn leaf color.