欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (23): 73-79.doi: 10.11924/j.issn.1000-6850.2012-1736

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

北极狐CETP基因序列扩增及结构特征分析

张敏 白秀娟   

  • 收稿日期:2012-05-07 修回日期:2012-06-07 出版日期:2012-08-15 发布日期:2012-08-15

Analysis of Arctic Fox's CETP Gene Order Augmentation and Structure Characteristics

  • Received:2012-05-07 Revised:2012-06-07 Online:2012-08-15 Published:2012-08-15

PDF (PC)

857

摘要：

为了研究北极狐胆固醇酯转运蛋白基因(CETP)的结构特征，找到影响北极狐体形、体重的重要基因，达到提高北极狐毛皮质量。对北极狐CETP基因序列扩增后得到的序列结构数据，利用SignalP 3.0 Server、ClustalX1.83、Mega 4.0、Promoter predictions、TMHM 2.0等生物学软件进行分析，获得了它的序列特征，并对其结构和功能进行了预测。结果表明：北极狐与犬的遗传距离是0.015为最近，与鼠的遗传距离是最远的，北极狐的胆固醇酯转运蛋白是一个分泌蛋白，主要是进行O糖基化修饰，蛋白的二级结构结果：α螺旋占34.87%，β转角占，18.97%，无规则蜷曲占46.15%。北极狐CETP基因是参与北极狐生产性能调控的重要候选基因。

关键词: 土壤含水量, 土壤含水量

Abstract:

o study the CETP gene structural features, we could found the important gene that impacts the Arctic fox's body and weight and purpose of improve the quality of the Arctic fox fur. By the Arctic fox cholesteryl ester transfer protein gene amplification and sequencing data, SignalP 3.0 and other biological to complete the analysis; by the bioinformatics analysis of the Arctic fox cholesterol ester transfer protein gene, we got its sequence features, and we also predicted its structure and function prediction. The results showed that the distance between Arctic fox's genetic and the dog's genetic was the most recent, that was 0.015, and that distance between Arctic fox was and the mouse was the farthest, whose transfer protein was a secreted protein. The modification O glycosylation was mainly undertaken. The results of protein secondary structure was alpha helix 34.87%, beta corner accounting for 18.97%, irregular twist accounting for 46.15%. The CETP gene was an important gene involved in the regulation of performance of Arctic fox.

中图分类号: