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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (15): 169-172.doi: 10.11924/j.issn.1000-6850.2012-1933

所属专题： 玉米

• 植物保护 农药 • 上一篇    下一篇

利用玉米秸秆培养木霉菌分生孢子的研究

张广志 李纪顺 扈进冬 张新建 杨合同 李红梅   

  • 收稿日期:2012-05-21 修回日期:2012-07-09 出版日期:2013-05-25 发布日期:2013-05-25
  • 基金资助:
    国家高技术研究发展计划(863计划)

Study on Utilizing Corn Stalk to Cultivate Conidiophore of Trichoderma spp.

  • Received:2012-05-21 Revised:2012-07-09 Online:2013-05-25 Published:2013-05-25

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摘要： 为了研究利用资源丰富的玉米秸秆作为发酵基质生产木霉分生孢子的可行性，本文研究了秸秆基质细度、含水量、外加营养成分及搅拌等因素对木霉分生孢子产孢量的影响。结果表明，细度适中，在40~10目之间，含水量60%~70%，利于木霉产孢,分生孢子产量可达1.27×1010 cfu/g(干培养物)；接种量对最终产孢量影响不大，处理间差异不明显；通过添加碳源葡萄糖，并加入少量(NH4)2SO4、CaCO3、KH2PO4和MgSO4无机盐，可显著提高木霉分生孢子的产量，分生孢子产量最高可达2.0×1010 cfu/g(干培养物)；但在固体培养过程中，对基质进行搅拌，不利于木霉分生孢子的形成。由此表明，利用玉米秸秆固体发酵生产木霉分生孢子可行、高效、无污染，对于木霉菌剂的生产和推广具有重要的参考价值。

关键词: 遗传多样性, 遗传多样性

Abstract: In order to research the Feasibility of Utilizing Corn Stalk, which was affluent but cheap, to process Conidial preparation of Trichoderma, fineness of corn stalk, moisture content, supplement of nutrient carbon sources and inorganic salts and stiring on the spore production were studied. The result showed that the fineness of corn stalk was 40-10 mesh, initial moisture was 60%-70%, and the spore production could reach 1.27×1010 spores per gram dry matter. Initial inoculum on the sporulation of Trichoderma had little impact. Add carbon sources and inorganic salts, the greatest spore production could reach 2.0×1010 spores per gram dry matter. But stiring was not conducive to sporulation. This showed that utilizing corn stalk to process conidial preparation of Trichoderma was feasible, efficiency, non-pollution, and it had important reference value to produce and promote Trichoderma agent.