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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (21): 41-46.doi: 10.11924/j.issn.1000-6850.2012-3407

所属专题： 玉米； 水稻

• 农学 农业基础科学 • 上一篇    下一篇

SSR标记技术在杂交水稻和杂交玉米种子质量鉴定中的应用研究

蒋晓英 杨春文 林清 吴红   

  • 收稿日期:2012-10-17 修回日期:2012-11-09 出版日期:2013-07-25 发布日期:2013-07-25
  • 基金资助:
    重庆市科技重点攻关项目“杂交水稻新品种DNA指纹库构建及应用研究”;重庆市农业发展资金项目（基于DNA水平的“两杂”作物种子质量监控技术体系的应用示范

The application of SSR marker in hybrid maize and hybrid rice seed quality assessment and purity test

  • Received:2012-10-17 Revised:2012-11-09 Online:2013-07-25 Published:2013-07-25

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摘要： SSR标记具有快速、稳定、准确的技术优势，已经在品种鉴定等方面得到广泛的应用。为了鉴定生产用“两杂”种子样品的遗传多样性及纯度，利用36对SSR引物对39份杂交水稻样品进行了遗传多样性鉴定，其中2个样品为“同名异种”（遗传距离为0.11），有2个样品疑似“异名同种”；利用33对SSR引物对36份杂交玉米样品进行了遗传多样性鉴定，同样存在“同名异种”和“异名同种”现象。在人为混杂的杂交水稻样本中，SSR标记检测值与实际混杂率u测验没有显著性差异；同时利用SSR标记和田间鉴定方法比较种子公司提供的10个杂交水稻样品纯度，尽管有3个样品SSR标记检测结果低于田间鉴定，但由于田间纯度鉴定以农艺性状为标准，遗传背景相似的单株难以区分，因此从加强种子质量监管角度考虑，SSR分子标记鉴定技术更为有利。

关键词: 发育, 发育

Abstract: SSR molecular markers which had the technical advantages of fast, stable and accurate were used to investigate the genetic diversity and purity test of two hybrid seed commercially available. 36 rice SSR primer pairs were used to assess the genetic diversity of 38 hybrid rice samples. The result showed two of the sample were heterogenous (genetic distance 0.11), whereas two of the others seemed to be synonym (genetic distance 0.03). 33 maize SSR primer pairs were used to determine the genetic diversity of 36 hybrid maize sample, the result showed two of the sample were heterogenous (genetic distance 0.12). In the artificial mixed sample test, u test revealed there was no significant difference between all sample test value and actual mixed ratio. Meanwhile, 10 hybrid rice samples provided by seed company were compared with SSR molecular marker and field identification methods, though SSR marker test results were below the field identification in three samples, the field identifying were on the basis of agronomic traits, it was difficult to distinguish the similar genetic background strains, it indicated that SSR molecular marker was more favorable than the other detection methods.