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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (10): 162-166.doi: 10.11924/j.issn.1000-6850.2012-3849

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

百合花青素苷合成酶基因片段的克隆及表达分析

王瑜 崔金腾 张克中 贾月慧   

  • 收稿日期:2012-11-27 修回日期:2012-12-17 出版日期:2013-04-05 发布日期:2013-04-05
  • 基金资助:
    北京市自然科学基金项目，“百合卷瓣组特异种质参与的杂交育种中花粉管定向生长与胚抢救研究”；北京市教委科技创新平台项目，“景观花卉创新平台建设”

Molecular Cloning and Expression Analysis of Anthocyanidin Synthase Gene Fragment in Lilium

  • Received:2012-11-27 Revised:2012-12-17 Online:2013-04-05 Published:2013-04-05

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摘要： 为了克隆百合花青素苷合成酶基因(anthocyanidin synthase, ANS)，通过已报道的其他物种的ANS基因保守序列设计简并引物，采用同源克隆的方法成功克隆得到了百合ANS基因片段，该片段长701 bp，编码233个氨基酸残基。根据蛋白比对结果，百合ANS基因编码的氨基酸序列与郁金香、荷兰鸢尾、甜樱桃的一致性分别为86%、81%、77%。采用半定量RT-PCR法分析表明，该基因在百合花瓣中的表达水平最高，叶和茎次之，鳞茎中不表达。本研究从百合中分离得到了ANS基因片段，为后续获得基因全长打下了基础。

关键词: 山西, 山西

Abstract: In order to obtain anthocyanidin synthase (ANS) gene from Lilium. degenerate primers were designed based on sequences blast of ANS genes from other species, and the fragment of ANS gene in Lilium was cloned by homology cloning method. The gene fragment was 701 bp encoding 233 amino acid proteins. Sequence alignment revealed that, the deduced amino acid sequence was 86%, 81% and 77% identical to Tulipa gesnerian, Iris hollandica and Prunus avium, respectively. The ANS expressed at different levels with the highest in petal, the second in leaf and stem. However, there’s no expression in bulb. ANS fragment was successfully cloned from Lilium through this research. This lays a solid foundation for cloning the full length cDNA sequence.there’s no expression in bulb.ANS fragment was successfully cloned from Lilium through tis research.This lays a solid foundation for cloning the full length cDNA sequence.