欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (24): 11-16.doi: 10.11924/j.issn.1000-6850.2013-0305(1)

所属专题： 水稻

• 农学 农业基础科学 • 上一篇    下一篇

贵港最大面积原生地新收集普通野生稻分子鉴定评价

  

  • 出版日期:2013-08-25 发布日期:2013-08-25

Molecular Identification of New Collecting Resources ofO. rufipogon Griff from the Largest Original Habitat in Guigang, Guangxi Province

  • Online:2013-08-25 Published:2013-08-25

PDF (PC)

231

摘要： 为了对来自贵港市最大面积原生地新收集普通野生稻资源进行遗传多样性分析，并在此基础上 进一步对居群内的个体进行区分。选择分布于水稻染色体组的25 对微卫星(SSR)引物对来自贵港的 349 份材料进行多样分析和遗传结构研究。结果表明，所有个体平均等位基因数A=6，有效等位基因数 Ae=2.23，期望杂合度He=0.44，实际杂合度Ho=0.40，香农指数I=0.88。该居群可分为4 个亚群结构，并 且90%以上个体可以得到区分。该群体遗传多样性丰富，SSR分子标记可以简单有效的区分群体内的 大部分个体，提高了保护效率。

关键词: 基因定位, 基因定位

Abstract: To estimate the genetic diversity of common wild rice from the Largest Original Habitat in Guigang, Guangxi and further to distinguish the individuals within the population. Twenty-five SSR loci distributed on the twelve chromosomes of rice were used to study the genetic diversity and structure of 349 wild rice individuals. The results showed that, the mean number of allele (A ) was 6 and the effective number of allele (Ae ) was 2.23. Expected heterozygosity (He ) was 0.44, and observed heterozygosity (Ho ) was 0.40 as well as the Shannon index (I ) was 0.88. The population could be divided into four subsets structure, and more than 90% of individuals could be distinguished. It had abundant genetic diversity within the population and SSR molecular markers was the effective method to distinguish most of individuals in the group also to improve protection efficiency of wild rice.