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中国农学通报 ›› 2014, Vol. 30 ›› Issue (13): 131-136.doi: 10.11924/j.issn.1000-6850.2013-2639

所属专题: 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

‘冬枣’子代种子萌发及下胚轴诱导愈伤组织研究

金琪 王岩 史良 王欢 张智勇 李颖岳   

  • 收稿日期:2013-10-10 修回日期:2013-10-22 出版日期:2014-05-05 发布日期:2014-05-05
  • 基金资助:
    林业公益性行业专项项目“枣新品种选育和产业升级关键技术研究项目”(201004017);北京林业大学科技创新计划项目“枣树多倍体种质创新研究”(YX2013-35)。

Seed Germination and Hypocotyl Callus Induction of Ziziphus jujuba ‘Dongzao’

  • Received:2013-10-10 Revised:2013-10-22 Online:2014-05-05 Published:2014-05-05

摘要: 为实施‘冬枣’遗传改良方案,以采自山西柳林自然授粉获得的‘冬枣’子代种子为材料,测量种子横、纵径、种仁率等基本形态指标,并进一步研究种子萌发最适条件和下胚轴诱导愈伤组织适宜培养基。结果表明:(1)‘冬枣’子代种子横径为(8.62±0.05) mm,纵径为(17.95±0.05) mm,千粒重为(54.27±4.62) g,种仁率为(64.17±3.82)%,‘冬枣’属于种仁率较高的枣树品种。(2)种仁在含有GA3 0.4 g/L的萌发培养基中预处理24 h后接种到萌发培养基上,最有利于种仁萌发,萌发率可达100%;液体萌发培养基稍好于固体萌发培养基,但未达显著水平。(3)下胚轴诱导愈伤组织的适宜培养基为1/2MS+ TDZ 1.0 mg/L+蔗糖30 g/L,诱导率为(60.00±3.02)%。

关键词: 重金属, 重金属, 镉污染, 铅污染, 甜高粱, 富集系数

Abstract: The seeds of Ziziphus jujuba Mill cv. Dongzao from Shanxi were collected as the materials. The seed morphological indexes, such as the transverse diameter and vertical diameter were measured, the thousand kernel weight (TKW) was weighted, and the rate of kernel was counted. Then the optimal condition for seed germination was studied and the appropriate medium for hypocotyl callus induction was selected. The results showed that: 1) the transverse, vertical diameter of the seed were (8.62±0.05)mm and(17.95±0.05)mm, respectively; TKW was (54.27±4.62)g; the rate of kernel was (64.17±3.82)%, so Dongzao was one of the high rate varieties of Ziziphus jujube. 2) the seeds on germination medium with GA3 0.4g/L for 24h pretreatment could reach the 100% germination rate; the liquid germination medium was a bit better than the solid, but the difference was not significant. 3) the best hypocotyl callus induction medium was 1/2MS+ TDZ 1.0mg/L+ sucrose 30g/L, and the induction rate was 60%.